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AAT Bioquest

What are the limitations of RNA interference (RNAi)?

Posted August 15, 2024


Answer

One limitation is the off-target effects. RNAi can sometimes affect unintended genes, leading to potential side effects. Another limitation is possible delivery issues. Introducing siRNA into cells effectively remains a challenge, although advancements in delivery reagents are improving this process. 

Additional resources

RNA Interference–Based Therapy and Its Delivery Systems

Gene Expression Analysis & Genotyping

Portelite™ Fluorimetric RNA Quantitation Kit*Optimized for Cytocite™ and Qubit™ Fluorometers*