What are the factors that can affect TRITC-dextran conjugation?

There are various factors that can affect TRITC-dextran conjugation. One factor is pH, and the optimal pH value for TRITC-dextran conjugation is 8.0. Temperature is another factor, as most reactions are conducted at room temperature. However, higher or lower temperatures may be used depending on the experiment. The method of conjugation is another factor. Direct coupling, Carbodiimide-mediated coupling, and crosslinking methods may cause different efficiencies and specificity of the reaction. Longer reaction times may cause a higher degree of conjugation, but may also cause non-specific binding or degradation of the conjugate. Higher concentrations of either component may cause protein aggregation.