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What are the disadvantages of indirect detection methods in immunohistochemistry (IHC)?
Posted May 2, 2024
Answer
- The binding between the secondary antibody and other proteins present on the blot can give rise to more non-specific signals.
- Additional incubation, blocking, and wash steps add time to the process.
Additional resources
Multiplex Spatial Protein Detection by Combining Immunofluorescence with Immunohistochemistry