Calculator
What are the disadvantages of direct detection methods in immunohistochemistry (IHC)?
Posted May 2, 2024
Answer
- The binding between the antibody and the target protein may be affected by the coupling of the label to the primary antibody.
- It is less sensitive than the indirect detection technique and may be better suited for applications in which the primary antibody is highly specific and there is a sufficient amount of target proteins.
- Every primary antibody needs to be labeled, which can add to the cost and time.
- It cannot be used for other applications down the line because the primary antibody is tagged with a label.
Additional resources