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AAT Bioquest

What are the different types of genetic markers?

Posted June 4, 2024


Answer

Types of genetic markers: 

  1. Random Amplified Polymorphic DNA (RAPD): RAPD uses a short arbitrary primer that serves as both, forward and reverse primer, to amplify random DNA segments without requiring prior DNA sequencing.
  2. Restriction Fragment Length Polymorphism (RFLP): RFLP involves using restriction enzymes to cut DNA at specific sites, resulting in fragments of varying lengths.
  3. Single Nucleotide Polymorphism (SNP): SNPs are single base pair variations in the DNA sequence, detected using SNP chips.
  4. Amplified Fragment Length Polymorphism (AFLP): AFLP combines PCR and restriction digestion to amplify selected DNA fragments.
  5. Sequence Characterized Amplified Region (SCAR): SCAR uses PCR with longer primers to produce highly reproducible markers.
  6. Cleaved Amplified Polymorphic Sequence (CAPS): CAPS involves PCR amplification followed by restriction digestion to reveal polymorphisms.
  7. Microsatellites or Simple Sequence Length Polymorphisms (SSLPs): Microsatellites or simple sequence repeated (SSR) loci are PCR-based markers that required previous knowledge of gene sequence. 
  8. Expressed Sequence Tags (EST): Expressed sequence tags are molecular markers synthesized by partial sequencing of random cDNA clones and used as tags for identification of genes. 
  9. Inter Simple Sequence Repeat (ISSR): ISSR uses PCR to amplify DNA segments between microsatellite repeat using a single primer with microsatellite core regions. 
Additional resources

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