What are the different types of antibodies available for use in ChIP assays?
Posted April 12, 2024
Answer
The different types of antibodies available for use in ChIP assays include: polyclonal antibodies, monoclonal antibodies, and epitope-tag antibodies.
- Monoclonal antibodies are generated by a single clone of B cells and recognize a specific epitope with high specificity.
- Polyclonal antibodies are produced by multiple clones of B cells and recognize multiple epitopes on the target protein.
- Epitope-tag antibodies are designed to recognize specific tags attached to the target protein. During immunoprecipitation, the protein of interest is typically expressed as a fusion protein with a tag inserted into either the N- or C-terminus.
The most suitable option for a ChIP or ChIP-seq experiment is an antibody that has been validated specifically for ChIP or ChIP-seq applications.
Additional resources
Chromatin Immunoprecipitation (ChIP)