What are the differences between super resolution and electron microscopy?

Question

Accepted Answer

Basis of differentiation

Super resolution microscopy

Electron microscopy

Definition

Is any type of microscopy which suprasses the resolution limit of typical light microscopy by at least a factor of two.

Is a microscope which uses accelerated beams of electrons (through electromagnetic lenses)

Resolution

Typically from 10-250 nm

0.2 nm

Mechanism

Uses different methods such as STED, SIM, PALM to surpass the diffraction limit of light

Uses beams of electrons to generate images

Field of view

In general has a comparatively larger field of view

Has a comparatively lower field of view

Speed

Imaging is typically fast (35 milliseconds in STED microscopy)

Takes longer due to sample preparation

Sample preparation

Is used in live-cell imaging and requires specialized fluorescent tags or dyes

Only dead and dried samples can be seen (requires sample fixation)

Basis of differentiation	Super resolution microscopy	Electron microscopy
Definition	Is any type of microscopy which suprasses the resolution limit of typical light microscopy by at least a factor of two.	Is a microscope which uses accelerated beams of electrons (through electromagnetic lenses)
Resolution	Typically from 10-250 nm	0.2 nm
Mechanism	Uses different methods such as STED, SIM, PALM to surpass the diffraction limit of light	Uses beams of electrons to generate images
Field of view	In general has a comparatively larger field of view	Has a comparatively lower field of view
Speed	Imaging is typically fast (35 milliseconds in STED microscopy)	Takes longer due to sample preparation
Sample preparation	Is used in live-cell imaging and requires specialized fluorescent tags or dyes	Only dead and dried samples can be seen (requires sample fixation)