Can super resolution imaging be used with live cells and tissues?

Yes, super resolution imaging can be used in live cells and tissues. Super structured illumination microscopy is a type of super resolution imaging that is ideal for live cell imaging, as it only requires 9 or 15 widefield images to generate one super-resolution image. The only limitations are that there should be minimal bleaching and movement throughout the process of achieving images. Structured-resolution microscopy is considered to be one of the best suited techniques for live cell imaging as it allows one to capture data for a single generated image in a timeframe between several seconds to 0.1s depending on the imaging system. Other types of super resolution microscopy such as localization microscopy have limitations such as their speed of capturing images, achieving up to ten thousand frames normally takes several minutes, and processes within cells typically occur in a much shorter amount of time than this.