What are the differences between PAGE and SDS-PAGE?
Posted July 17, 2024
Basis of differentiation |
PAGE |
SDS-PAGE |
Definition |
Is a laboratory technique that separates proteins based on their 3D conformation (size, shape, and charge) |
Is a laboratory technique that separates proteins based on their molecular weight or mass |
Gel feature |
Uses non-denaturing gel |
Uses denaturing gel |
Presence of SDS in Gel |
SDS is absent |
SDS is present as a detergent to impart a negative charge on the sample |
Separation criteria |
Proteins are separated based on molecular size and overall charge |
Proteins are separated based on molecular weight |
Sample preparation |
Protein samples are not heated |
Protein samples are heated |
Buffer composition |
Reducing agent is not present in the buffer |
Buffer has a reducing agent such as BME or DTT |
Temperature |
Is run at 40°C |
Is run at room temperature |
Net charge on proteins |
May be either positive or negative |
Is always negative |
State of protein |
Native conformation or folded state |
Denatured |
Protein stability |
High stability |
Low stability |
Recovery of original protein |
Can be recovered after separation |
Cannot be recovered after separation as it is denatured during the process |
Protein function |
Proteins retain their function |
Proteins lose their function |
Ease of use |
Comparatively difficult resulting in infrequent usage |
Easier to perform and is used frequently |
Applications |
Is used to:
|
Is used to:
|
Speeding up SDS-PAGE: Theory and experiment