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AAT Bioquest

What are the advantages of SDS-PAGE electrophoresis?

Posted July 17, 2024


Answer

Advantages of SDS PAGE

  • Involves a simple operation that simplifies more complex tasks 
  • Enables detection of specific proteins based on mass and characters
  • Enables identification of different species based on mass of the protein
  • Offers high resolution and advanced separation of protein molecules based solely on mass
  • Enables determination of molecular weight of protein in a sample
  • Analyzes post-translational modifications
  • Is used in peptide-mapping, estimating protein size, identifying purity of proteins, and separating HIV proteins in HIV tests
Additional resources

Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis of Proteins

Gel Electrophoresis

SDS-PAGE SDS Running Buffer (10x) Preparation and Recipe

Gel Electrophoresis Annotator