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AAT Bioquest

What are the differences between gel and capillary electrophoresis?

Posted May 29, 2024


Answer

Basis of differentiation

Gel electrophoresis

Capillary electrophoresis

Definition

Is a method which separates molecules on a plane using a polymer gel medium

Is a technique which separates molecules on a capillary tube using a liquid or gel polymer medium

Cross-linkage

Displays low resolution

Displays higher resolution

Detection method

Detection is carried out by staining and observing through UV transilluminator

Detection is carried out through spectrophotometric automated detectors 

Surface Area to Volume Ratio

Has a lower surface area to volume ratio

Has a higher surface area to volume ratio

Additional resources

Comparison between agarose gel electrophoresis and capillary electrophoresis for variable numbers of tandem repeat typing of Mycobacterium tuberculosis

Gel Electrophoresis

Gelite™ Green Nucleic Acid Gel Staining Kit