What are the differences between forward primer and reverse primer?

Forward primer and reverse primer are two types of primers used in the process of PCR (polymerase chain reaction). There are a few notable differences between the two. 

Forward Primer

• Forward primers are DNA segments that are complementary to the antisense strand of double-stranded DNA. 
• They are also known as 5′ primers because they occur at the 5′ end of the PCR product. 
• Forward primers bind to the antisense strand (-) of the DNA that runs in 3′ to 5′ direction. They are responsible for the amplification of the antisense strand.
• Forward primers anneal with the template strand to facilitate the synthesis of the coding sequence.

Reverse Primer

• Reverse primers are DNA segments that are complementary to the sense strand of the double-stranded DNA.
• They are also known as 3′ primers because they occur at the 3′ end of the PCR product. 
• Reverse primers bind to the sense strand (+) of the DNA that runs in the 5′ to 3′ direction. They are responsible for the amplification of the sense strand. 
• Reverse primers anneal with the non-template strand to facilitate the synthesis of noncoding sequences