What are the differences between primers and promotors?
Posted May 12, 2023
Basis of differentiation |
Primers |
Promoters |
Definition |
Primers commercially synthesized short DNA sequences used to amplify target DNA sequences in polymerase chain reaction (PCR). |
Promoters are specific regulatory DNA sequences that are found upstream of the transcription initiation site of a site. They provide a secure initial binding site for RNA polymerase and transcription factors to facilitate transcription. |
Length |
Primers have about 20 base pairs. |
Promoters can have about 100-1000 base pairs. |
Function |
Primers serve as starting sequences for the synthesis of a new strand. |
Promoters regulate gene transcription by providing binding sites for RNA polymerase and other transcription factors. They define the direction of the transcriptions and indicate the sense strand of a gene. |
Types |
2 types - Forward primers - Reverse primers |
3 types - Core promoters - Proximal promoters - Distal promoters |
Sequence |
Primers have nucleotide sequences that are complementary to the flanking ends of the DNA double-strand (target) |
Promoters have specific DNA sequences |
Polymerase Chain Reaction (PCR)