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AAT Bioquest

What are the differences between continuous and synchronous culture?

Posted December 15, 2023


Answer

Basis of differentiation

Continuous culture

Synchronous culture

Definition

Is a set of methods used to consistently grow microorganisms under controlled growth rates for extended periods

Are cultures in which the majority of organisms are in the same stage of the cell cycle  

Nutrients

Nutrients are constantly added

Nutrients are not added continuously

Cell cycle

Does not stay in same stage of the cell cycle

Stays in the same stage of the cell cycle

Purpose

Its main purpose is to retain a continuous flow between the addition of fresh media and removal of cell suspension

Its main purpose is to cause the growth and division of cells so that majority of them pass through specific phases of the cell cycle 

Additional resources

Continuous culture

Cell Proliferation Assays

Assay development services

HHBS [Hanks' Buffer with 20 mM Hepes]