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AAT Bioquest

What are the differences between absorbance and transmittance?

Posted December 15, 2022


Answer

Basis of differentiation

Absorbance

Transmittance

Definition

Measures the amount of incident light that is absorbed when it travels through a material

Measures the amount of light passing through a sample

Range

Values range from 0 to 1 

Has values from greater than 0 and higher 

Formula

A = Log 10 (i0/i)

T = i/i0

Value change as concentration is increased

Decreases exponentially 

Increases linearly 

Inter-relation 

When all light is completely absorbed by the sample, absorption is 100% and transmittance is 0% 

If all light passes through a sample, its transmittance would be 100% and absorption 0%

Additional resources

Beer's Law‐Why Integrated Absorbance Depends Linearly on Concentration

Absorption Spectrum Viewer