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AAT Bioquest

Posted on May 6, 2024
What is UV-Vis spectrophotometer?
AbsorbanceFluorescencespectrophotometer


Posted on May 6, 2024
How does a UV-Vis spectrophotometer work?
AbsorbanceFluorescencespectrophotometer


Posted on May 6, 2024
How do I choose the right wavelength of the spectrophotometer for my experiment?
AbsorbanceFluorescencespectrophotometer


Posted on May 6, 2024
What is a spectrophotometer?
AbsorbanceFluorescencespectrophotometer


Posted on May 6, 2024
How does a spectrophotometer work?
AbsorbanceFluorescencespectrophotometer


Posted on May 6, 2024
What is a spectrophotometer used for?
AbsorbanceFluorescencespectrophotometer


Posted on May 6, 2024
What are the commonly used wavelength selectors in the spectrophotometer?
AbsorbanceFluorescencespectrophotometer


Posted on May 6, 2024
What are the advantages of UV-Vis spectroscopy?
AbsorbanceFluorescencespectrophotometer


Posted on May 6, 2024
What are the limitations of UV-Vis spectroscopy?
AbsorbanceFluorescencespectrophotometer


Posted on May 6, 2024
What are the applications of UV-Vis spectroscopy?
AbsorbanceFluorescencespectrophotometer


Posted on January 4, 2024
What is absorption?
Antibodies and Proteomics Bioconjugation Chemical Reagents ToolsAbsorbanceMeasure absorbance


Posted on January 13, 2023
What are the differences between absorbance and fluorescence?
Chemical ReagentsAbsorbanceFluorescenceMeasure absorbancespectrophotometer


Posted on December 15, 2022
What are the differences between absorbance and transmittance?
AbsorbanceBeer-Lambert LawMeasure absorbanceSpectral Flow Cytometry


Posted on December 15, 2022
What does a high percent transmittance mean?
Absorbancespectrophotometer


Posted on December 15, 2022
What are the applications of transmittance?
Absorbancespectrophotometer


Posted on December 15, 2022
How is absorbance calculated from transmittance?
AbsorbanceBeer-Lambert LawMeasure absorbance


Posted on November 2, 2020
What factors will affect the molar extinction coefficient value?
AbsorbanceMolar Extinction Coefficient


Posted on October 30, 2020
What factors will affect absorbance?
AbsorbanceMeasure absorbanceSpectral Flow CytometryspectrophotometerBeer-Lambert Law


Posted on June 1, 2020
Why is fluorescence more sensitive than UV-VIS absorption spectroscopy?
Chemical ReagentsAbsorbanceFluorescenceMeasure absorbancespectrophotometer


Posted on April 24, 2020
What is the difference between absorption and excitation spectra?
AbsorbanceMeasure absorbanceexcitation wavelengthspectrophotometer


Posted on April 17, 2020
What is the difference between absorbance and absorption?
AbsorbanceBeer-Lambert LawEnergyMeasure absorbancespectrophotometer


Posted on April 17, 2020
What is absorbance?
AbsorbanceBeer-Lambert LawMeasure absorbancespectrophotometer


Posted on April 17, 2020
What is OD?
AbsorbanceBeer-Lambert LawMeasure absorbancespectrophotometer


Posted on April 17, 2020
What is the difference between OD and Abs?
AbsorbanceBeer-Lambert LawMeasure absorbancespectrophotometer


Posted on February 19, 2020
Why is my NADH assay showing unreasonably high absorbance readings?
AbsorbanceCell AssayColorimetricNADH AssayNADNADHNADPNADPHStock Solutionbufferenzymenicotinamide adenine dinucleotidenicotinamide adenine dinucleotide phosphatespectrophotometer


Posted on February 4, 2020
What methods are currently used to quantify DNA?
DNA quantificationAbsorbanceFluorescenceAgarose gel electrophoresis


Posted on October 22, 2019
What are the differences between MTT and WST assays?
AbsorbanceCell ViabilityCell AssayCell CytotoxicityColorimetricWST-8cell proliferation


Posted on June 13, 2019
How do you prepare cell samples for a sphingomyelinase assay?
SphingomyelinaseAbsorbancemicroplate reader


Posted on April 2, 2019
How do I prepare a spin column?
AbsorbanceCalculating ConcentrationConjugationPBS


Posted on April 2, 2019
Why should you use ABTS when measuring the concentration of an antioxidant in a biological sample?
AbsorbanceABTSH2O2HRPROScolorimetric


Posted on April 2, 2019
Does EDTA interfere with your Cell Meter™ Colorimetric Antioxidant Activity Assay Kit (Cat # 15900)?
AbsorbanceH2O2HRPABTSCell Assay


Posted on July 8, 2018
How do you determine the concentration of peridinin-chlorophyll protein complex (PerCP)
AbsorbancePerCPCalculating ConcentrationPeridinin-Chlorophyll Protein Complex


Posted on January 17, 2018
How do you determine the concentration of PE [R-Phycoerythrin] (Cat#2258) using a NanoDrop™ 2000 Spectrophotometer?
PhycoerythrinCalculating ConcentrationAbsorbanceProtein Concentration Calculator