What are the common variables that increase molecule passage?

Common variables that increase molecule passage: 

• Differences between linear and globular molecules 
• Low sample concentration below 1 mg/mL 
• Buffer composition that promotes the disintegration of molecules, influenced by pH and ionic conditions that change the molecule’s conformation or aggregation properties
• Elevated transmembrane pressure induced by gravitational force in centrifugal concentrators - particularly impactful for linear molecules such as DNA fragments. Lowering the g-force can enhance the membrane’s molecule retention.