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What are the common amine-reactive moieties?

Posted May 23, 2024


Antibodies and Proteomics
Bioconjugation
Antibody and Protein Labeling
Dyes by Functional Group
Answer

Common amine-reactive moieties include isothiocyanates, epoxides, succinimidyl esters, acyl azides, anhydrides, and NHS esters. These moieties typically react with amines through either alkylation or acylation processes.

Additional resources

Amine-Reactive Activated Esters of meso-CarboxyBODIPY: Fluorogenic Assays and Labeling of Amines, Amino Acids, and Proteins

Amine Reactive Dyes and Probes for Conjugation

iFluor® 488 succinimidyl ester

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