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AAT Bioquest

What are the classifications of microarray based on the mode of preparation?

Posted September 11, 2024


Answer

Microarrays are classified into 3 types based on the mode of preparation. 

  1. Spotted arrays on glass are created on glass slides coated with poly-lysine, which helps DNA stick to the surface. DNA is deposited using specialized pins, and fluorescent labels can be used to mark the samples. 
  2. In-situ synthesized arrays are made directly on a solid surface using chemical synthesis. This process combines special light-sensitive chemicals with photolithography techniques to create the array. 
  3. Self-assembled arrays involve fiber optic arrays where DNA is attached to small polystyrene beads. The beads are placed onto the ends of a fiber optic cable, with different DNA types on different beads. When a mixture of these beads is applied, they form a randomly assembled array.
Additional resources

Basic Concepts of Microarrays and Potential Applications in Clinical Microbiology

Gene Expression Analysis & Genotyping

Helixyte™ Green Fluorimetric dsDNA Quantitation Kit *Optimized for Broad Dynamic Range*