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AAT Bioquest

What are the benefits of using agarose beads for immunoprecipitations?

Posted April 12, 2024


Answer

There are several benefits of using agarose beads for immunoprecipitation experiments. Two key benefits are they offer high capacity and yield. 

  • Agarose resin beads have a sponge-like structure with a large surface area, providing a high binding capacity for antibodies. In contrast, magnetic beads, while smaller and less porous, have a lower binding capacity. 
  • Additionally, agarose beads can be manipulated with simple equipment like centrifuges or filtration systems, making them accessible for a wide range of labs. They are also more cost-effective in the short term compared to magnetic beads.
Additional resources

Immunoprecipitation

Immunoprecipitation

ReadiPrep™ Protein A-Agarose Resin

Antibody & Protein Conjugation Service