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AAT Bioquest

What are the advantages of tank transfer technique?

Posted April 10, 2024


Answer

Tank transfer transfer, which is particularly advantageous for transferring large proteins, is less susceptible to membrane drying issues and less susceptible to membrane drying issues. Tank transfer is the preferred method for transferring negatively charged polypeptides from an SDS-PAGE gel to a membrane blot. Additionally, the equipment for this method is cost-effective. Using a self-contained cooling apparatus reduces energy consumption compared to refrigerated space. 

Additional resources

Western Blotting: An Introduction

Western Blotting Assays

iFluor® 488 goat anti-rabbit IgG (H+L)