What are the advantages of Chemiluminescent detection method for western blot?

One advantage is that by utilizing digital cameras, chemiluminescent detection allows for the identification of membranes where the signal intensity may exceed the optimal range. This ensures that measurements are within the optimal detection range, facilitating precise quantification of protein levels. Another advantage is that chemiluminescent detection methods enable the detection and quantification of extremely small amounts of protein (even as low as femtograms) by utilizing specialized HRP substrates which react with the protein of interest and produce light.