What are the different types of transfer methods in western blot?

Different types of transfer methods in western blot include wet transfer, diffusion transfer, electroblotting, vacuum blotting, and capillary transfer.

• In diffusion transfer, the gel and membrane are placed together with an absorbent pad soaked in transfer buffer. Proteins passively diffuse from the gel onto the membrane over time. 
• In vacuum blotting, the gel and membrane are assembled in a blotting apparatus, and a vacuum is applied to draw the transfer buffer through the gel and onto the membrane. 
• Electroblotting (also known as electrotransfer) involves the use of an electric field to drive proteins from the gel onto the membrane. The gel and membrane are sandwiched between electrode plates, and an electric current is applied. Proteins migrate out of the gel and onto the membrane according to their charge and size. 
• Capillary transfer involves placing the gel and membrane in close contact with each other and sandwiching them between layers of absorbent paper soaked in transfer buffer.