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AAT Bioquest

What are the advantages of organic dyes in FRET?

Posted May 15, 2024


Answer

Organic dyes possess wide and adjustable absorption spectra and strong fluorescence properties. Thus, these dyes exhibit high fluorescence quantum yields, resulting in strong and detectable signals (bright colors). Organic dyes are also commercially available in a wide range of colors and properties, making them accessible for researchers and facilitating the design of custom FRET experiments.

Additional resources

Quantum dots versus organic dyes as fluorescent labels.

Förster Resonance Energy Transfer (FRET)

Screen Quest™ TR-FRET No Wash cAMP Assay Kit

FMOC-Asp(5-FAM)-OH