What are the advantages of fluorescent protein fluorophores in FRET?

One advantage is that fluorescent proteins can be genetically encoded and fused to proteins of interest, allowing for specific labeling of target molecules within cells. There is a wide variety of fluorescent proteins available with different spectral properties, allowing for flexibility in designing FRET pairs designed to specific experimental needs. Fluorescent proteins are also compatible with live-cell imaging techniques, making them suitable for studying cellular processes in real time. Another advantage is that fluorescent proteins are relatively stable, allowing them to be used in long-term imaging studies.