What are some examples of selective and differential media?
Posted December 22, 2023
Answer
Examples of selective media include: eosin methylene blue, MacConkey agar, Terrific Broth, YM (yeast and mold), hektoen enteric agar, xylose lysine, and Mannitol salt agar.
- Eosin methylene blue inhibits Gram-positive bacteria due to the presence of methylene blue, permitting only the growth of Gram-negative bacteria.
- YM has a low pH, hampering the growth of bacteria.
- Xylose lysine is selective for Gram-negative bacteria.
- Mannitol salt agar encourages the growth of Gram-positive bacteria and is distinctive for mannitol.
- Terrific Broth with glycerol is used specifically for growing recombinant strains of E. coli.
- Hektoen enteric agar is designed for Gram-negative bacteria.
Examples of differential media include: blood agar, X-gal plates, Streptococcuseosin methylene blue, and MacConkey agar.
- Blood agar contains bovine heart blood which becomes see-through when hemolytic activity occurs.
- X-gal plates are used to identify lac operon mutants.
- MacConkey agar helps differentiate bacteria based on their capacity for lactose fermentation.
- Streptococcuseosin methylene blue distinguishes bacteria based on its ability to ferment lactose and sucrose.
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