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AAT Bioquest

What are FRET-based immunosensors?

Posted May 15, 2024


Answer

FRET-based immunosensors are analytical tools that utilize FRET to detect and quantify specific antigens or antibodies in a sample. These sensors typically involve the use of fluorescently labeled antibodies or antigens, where one fluorophore acts as a donor and the other as an acceptor. When the target antigen binds to its corresponding antibody it brings the donor and acceptor fluorophores into close proximity.  Energy transfer then occurs, leading to a measurable change in fluorescence signal.

Additional resources

Towards a FRET-based immunosensor for continuous carbohydrate monitoring

Förster Resonance Energy Transfer (FRET)

Screen Quest™ TR-FRET No Wash cAMP Assay Kit

FMOC-Asp(5-FAM)-OH