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AAT Bioquest

What advantages does FACS have over MACS?

Posted May 9, 2023


Answer

FACS (fluorescence-activated cell sorting) and MACS (magnetic-activated cell sorting) are two types of cell sorting techniques. Each method has its own advantages and disadvantages. 

FACS has several advantages over MACS:

  • It is more versatile and allows for more in-depth isolations, allowing researchers to separate cells based on their size, granularity, and surface markers. 
  • It can measure a larger number of parameters on the same sample.
  • It collects information from millions of cells in a few seconds.
  • It yields higher purity. 
Additional resources

Methodological comparison of FACS and MACS isolation of enriched microglia and astrocytes from mouse brain

Fluorescence Activated Cell Sorting (FACS)

ReadiUse™ CFSE [5-(and 6)-Carboxyfluorescein diacetate, succinimidyl ester] *CAS 150347-59-4*