What advantages do FRET-based pH sensors have over traditional pH sensors?

FRET-based pH sensors provide several benefits compared to conventional pH sensors. They offer increased sensitivity and specificity, allowing for more accurate detection of pH changes, even in complex samples. Additionally, they enable real-time monitoring of pH variations in living organisms using fluorescence imaging methods. This capability is valuable for gaining knowledge about biological processes and discovering potential targets for therapeutic treatments. FRET-based biosensors have applications in monitoring water treatment processes like ion exchange, demineralization or reverse osmosis. They can detect the presence of hard water ions in treated water, allowing for efficient assessment of water treatment efficiency.