Should cells be equilibrated before a flow calcium assay?

Yes, it is standard for cells to be equilibrated before a flow calcium assay. Equilibrating cells before a flow calcium assay is a crucial step to ensure accurate measurements of intracellular calcium levels. Equilibration allows cells to adapt to the experimental environment, ensuring that they are under stable conditions before the assay begins. Before collecting data on the flow cytometer, it is recommended to equilibrate samples at 37°C for approximately 10 minutes.