Can I use the same calcium reagents for microscopy as I do for flow?

Yes, the same calcium indicators or reagents can be used for both microscopy and flow cytometry. Calcium-sensitive dyes are commonly used in both imaging techniques. These dyes allow researchers to visualize and quantify changes in intracellular calcium levels. It is important to ensure that the reagent is compatible with both types of techniques, as some dyes or fluorophores may be more suited for other imaging methods. Examples of fluorophores that can be used in microscopy and flow cytometry are GFP, rhodamine, and fluorescein isothiocyanate.