How is WGA typically conjugated for us in experiments?

WGA is commonly conjugated with a fluorescent dye such as fluorescein isothiocyanate (FITC) for visualization of structures under fluorescence microscopy. WGA can also be conjugated with biotin, allowing for detection through streptavidin-biotin complex formation. This method is commonly used in immunofluorescence. Additionally, WGA can be conjugated with enzymes such as horseradish peroxidase (HRP) for use in ELISA assays. This enables the detection of WGA-labeled structures through enzymatic reactions that produce colorimetric signals.