How do I make BSA for BCA assay?

The steps for making BSA for BCA assay are described below.

1. Prepare the BSA Stock Solution: Dissolve 10 mg of BSA (bovine serum albumin) in 10 mL of distilled water to create a 1 mg/mL stock solution.
2. Create BSA Standard Solutions: From this stock solution, prepare a series of BSA standard solutions with concentrations ranging from 0 to 2 mg/mL. Use distilled water to make the necessary dilutions for these standards.
3. Prepare the BCA Working Reagent: Mix 50 parts of BCA Reagent A with 1 part of BCA Reagent B. Ensure thorough mixing and allow the reagent to incubate at room temperature for 30 minutes before use.
4. Setup the Assay: Pipette 10 μL of each BSA standard solution and the protein samples into separate wells of a 96-well microplate.
5. Add the Working Reagent: Add 200 μL of the prepared BCA working reagent to each well.
6. Mix and Incubate: Mix the contents of each well by pipetting or gently shaking the plate. Incubate the plate at 37°C for 30 minutes.
7. Cool and Measure: Following incubation, allow the plate to cool to room temperature. Measure the absorbance of each well at 562 nm using a microplate reader. For best results, measure the absorbance within 30 minutes of stopping the reaction.
8. Analyze Results: Plot a standard curve using the absorbance values of the BSA standards. Use this standard curve to determine the protein concentration of the samples.