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AAT Bioquest

What are the advantages of BCA assays?

Posted September 30, 2024


Answer

One key pro of BCA assays is the stability of the color complex it forms. Once the purple color is developed, it remains stable for an extended period. Another pro is the BCA assay is able to be used over a broad range of protein concentrations. Additionally, the BCA assay is compatible with a broad spectrum of ionic and non-ionic detergents, as well as denaturing agents. Another advantage is the assay's reduced susceptibility to detergents. Furthermore, its high sensitivity allows the assay to detect small amounts of protein. The BCA assay is also effective for analyzing protein yields in whole cell lysates and affinity column fractions. This assay also provides greater protein-to-protein uniformity. 

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