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AAT Bioquest

How do I calculate the osmolarity of a solution?

Posted May 31, 2024


Answer

When calculating the osmolarity of solutions containing only one type of solute, one should use the equation that involves the solute's molarity, the number of particles it dissociates into (n), and a factor called the osmotic coefficient (f). This equation is osmolarity = molarity x n x f.  

For solutions with multiple solutes, the osmolarity can be found by finding the total sum of the molarities of each solute in a solution. The osmotic coefficient reflects how the solute behaves in solution. For electrolytes and other substances, it can be smaller or greater than one, but it tends to approach one as the solution becomes more dilute. These coefficients are determined through experiments measuring properties like osmotic pressure, solvent vapor pressure or boiling point.

Additional resources

How to Calculate the Osmolarity

Molarity Calculator

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