Can I run multiplex immunohistochemistry (IHC) using fluorescent detection?

Yes, multiplexing can be achieved through using fluorescent detection. In fluorescent detection, antibodies are linked with fluorochromes and emit light when exposed to a shorter wavelength of light. Depending on the immunoassay method used, it's possible to measure up to 20 or more different substances in a single sample. Fluorescent staining offers a wider range of colors and narrow emission spectra for each color, allowing for the detection of multiple targets with minimal spectral overlap. Fluorescent detection also provides the advantage of better identifying co-localized targets. Researchers  use fluorescent western blotting, fluorescence-linked immunosorbent assays (FLISAs), spectral flow cytometry and a wide array of bead-based fluorescent immunoassays for fluorescent detection.