What is the pKa of the phosphate group in DNA and RNA molecules?
Posted January 26, 2021
Answer
The phosphate groups in DNA and RNA molecules have a pKa of approximately 2. This is largely due to the fact that at a physiological pH the phosphate groups in the sugar-phosphate backbone are fully ionized
Additional resources
Experimental approaches for measuring pKa's in RNA and DNA
PhosphoWorks™ Fluorimetric Phosphate Assay Kit *Red Fluorescence*