What are the three stages of translation?
Posted June 22, 2020
Answer
Translation of an mRNA molecule occurs in three stages: initiation, elongation, and termination.
- Initiation: The ribosome assembles around the target mRNA and the start codon 5’ AUG is recognized.
- Elongation: The tRNA transfers an amino acid to the tRNA bound to the next codon, forming a peptide bond between the two amino acids. The ribosome then translocates to the next codon to continue the process, creating an amino acid chain in the direction from the N terminal to the C terminal.
- Termination: When a stop codon is recognized, the elongation of amino acid chain terminates. The ribosome then folds the polypeptide into its final structure.
Additional resources
Helixyte™ Green *10,000X Aqueous PCR Solution*
6-ROX glycine *25 uM fluorescence reference solution for PCR reactions*