What are the advantages of fragment analysis?
Posted March 25, 2022
Answer
Fragment analysis is a genetic analysis technique that offers several advantages including:
- High sensitivity – Allows researchers to accurately size fragments differing by only one base pair
- Automated workflows – Allows researchers to run thousands of DNA fragment samples in one day
- Easy data analysis – The genetic analysis software simplified data analysis
- Simple preparation and cleanup – There’s no need to perform a DNA cleanup as opposed to other similar techniques such as sequencing
- Multiplexing capability – Labeling locus-specific primers with different colored dyes makes it easier to distinguish alleles for overlapping loci, enabling the analysis of more than twenty loci in a single reaction
- Ability to achieve high throughput – This can be done using capillary arrays
- Ability to obtain relative quantitation information from peak intensity measurements
- Independent method – Knowledge of the sequence of the fragment is not required
Additional resources
Digital fragment analysis of short tandem repeats by high-throughput amplicon sequencing