What are some methods for conjugation of antibodies with metallic nanoparticles?

Posted April 24, 2020

Answer

One method of conjugating antibodies with metallic nanoparticles is through passive adsorption, which involves coating the nanoparticle with citrate. At the proper pH and conditions, the antibodies should spontaneously attach to the citrate-coated surface of the nanoparticle.

Antibody and nanoparticle conjugation can also be done by binding functional groups such as amine groups to the surface of the nanoparticle. The covalent bond that forms between the functional groups and antibodies allows for exceptional stability.

Additional resources

Jazayeri, M.H., Amani, H., Pourfatollah, A.A., Pazoki-Toroudi, H., & Sedighimoghaddam, B. (2016). Various methods of gold nanoparticles (GNPs) conjugation to antibodies. Sensing and bio-sensing research, 9, 17-22. doi:10.1016/j.sbsr.2016.04.002

Oliveira, J.P., Prado, A.R., Keijok, W.J., Antunes, P.W., Yapuchura, E.R., & Guimarães, M.C. (2019). Impact of conjugation strategies for targeting of antibodies in gold nanoparticles for ultrasensitive detection of 17β-estradiol. Scientific Reports, 9. doi:10.1038/s41598-019-50424-5

Bioconjugation