Is your Intracellular Total ROS Activity Assay Kit Green Fluorescence wash free?

Posted August 8, 2018

Can I directly add 100uL of the Amplite ROS Green working solution to 100uL of cells? Or do I need to wash the cells or remove the medium before adding the working solution?

ROS

cell assay

no wash

fluorescent plate reader

live cell assay

Answer

No, you don't need to wash the cells or remove the medium. Simply add 100uL of the Amplite ROS Green working solution directly to 100uL of cells.

Additional resources

Cell Meter™ Fluorimetric Intracellular Total ROS Activity Assay Kit*Green Fluorescence*

References & Citations