How do I prepare a spin column?

I'd like to use a 1 mL spin column, such as a Sephadex^® G-25, BioGel^® P-6 DG Media or other desalting column to purify 100 µL of a reaction mixture. Can you provide a sample protocol?

Below is a sample procedure on how to set up and use a 1 mL spin column for purification of 100 µL test conjugate:

1. Fill with beads
2. Drain the solution, top dry
3. Buffer exchange using ~4 mL PBS buffer (pH 7.2 - 7.4)
4. Spin column at 2000X for 2 minutes
5. Exchange current 15 mL tube for a brand new one
6. Load sample mixture directly to the top of the desalting column
7. Add 10 µL of PBS buffer (pH 7.2 - 7.4)
8. Spin column at 2000X for 5 minutes
9. Collect elution
10. Measure the concentration using a spectrophotomer