Hypochlorite Assay

Hypochlorite anion (ClO^-) and its protonated form, hypochlorous acid (HClO) are critical reactive oxygen species (ROS) in biological systems. Uncontrolled production of hypochlorite (hypochlorous acid) can lead to tissue damage and diseases including arthritis, renal failure and cancers. In addition, sodium hypochlorite (NaClO) has been widely used as a bleaching agent for surface cleaning, odor removal and water disinfection in our daily lives. Exposure to large amount of sodium hypochlorite can lead to poisoning with the symptoms of serious breathing problems, stomach irritation, redness and pain on skin and eye.

Amplite^® Fluorimetric Hypochlorite (Hypochlorous Acid) Assay Kit (Cat# 13846) offers a sensitive fluorescence-based assay for measuring hypochlorite (hypochlorous acid) with high specificity. Upon selective reaction with hypochlorite (hypochlorous acid) the weakly fluorescent Oxirite™ Hypochlorite Sensor generates a strongly fluorescent product that gives more than 100-fold fluorescence enhancement. The fluorescence signal can be measured by a fluorescence microplate reader at Ex/Em= 540/590 nm. With Amplite^® Fluorimetric Hypochlorite (Hypochlorous Acid) Assay Kit, as low as 3 µM of hypochlorite was detected in a 100 µL reaction volume.

Amplite^® Colorimetric Hypochlorite (Hypochlorous Acid) Assay Kit (Cat# 13845) uses Oxirite™ Hypochlorite Sensor, which selectively reacts with hypochlorite (hypochlorous acid) to generate a red color product. The assay can be measured with an absorbance microplate reader at around 550 nm.