Catalase Detection

Catalase is a common antioxidant heme-containing redox enzyme found in nearly all living organisms that are exposed to oxygen. The enzyme is concentrated in the peroxisome subcellular organelles. Hydrogen peroxide is a reactive oxygen species (ROS) that is a toxic product of normal aerobic metabolism and pathogenic ROS production involving oxidase and superoxide dismutase reactions. By preventing the excessive buildup of H₂O₂, catalase allows important cellular processes which produce H₂O₂ as a by-product to take place safely

Amplite^® Fluorimetric Catalase Assay Kit (Cat# 11306) provides a quick and sensitive method for the measurement of catalase activity. Catalase reacts with H₂O₂ to produce water and oxygen (O₂). Amplite^® Red used in the assay kit reacts with H₂O₂ to generate a red fluorescent product. Therefore the reduction in fluorescence intensity is proportional to catalase activity. Amplite^® Red enables a dual recordable mode. The fluorescent signal can be easily read by either a fluorescence microplate reader at Ex/Em = 540/590 nm or an absorbance microplate reader at ~576 nm. With Amplite^® Fluorimetric Catalase Assay Kit, as low as 30 mU/mL catalase was detected in a 100 µL reaction volume.