iFluor™ 700 Dyes

Spectrally similar to Alexa Fluor^® 700 dyes, iFluor^® 700 dyes have fluorescence emission maximum at 710 nm with fluorescence quantum yields close to 0.2. Compared to Alexa Fluor^® 700 dyes, iFluor^® 700 dyes are brighter with stronger absorption at 633 nm. Fluorescence emission of iFluor^® 700 dyes is well separated from that of other commonly used red fluorophores, such as TAMRA, R-phycoerythrin and iFluor^® 647 dyes, making it ideal for three or four-color labelings. iFluor^® 700 dyes can be effectively excited by the 633 nm red laser in flow cytometers, giving additional near infrared emission. iFluor^® 700 dyes are also excellent acceptor dyes for allophycocyanin (APC), further facilitating multicolor flow cytometry analysis. Our in-house research indicated that the iFluor^® 700-labeled antibodies generally give much higher stain index than the corresponding antibodies labeled with Alexa Fluor^® 700 (See Figure 2).