Glycerol Assays

Glycerol is a precursor for synthesis of triglycerides and phospholipids in liver and adipose tissue. When fasting, triglycerides stored in these lipid droplets can be hydrolyzed to generate free glycerol and fatty acids. The amount of free glycerol released to the bloodstream is proportional to the triglyceride/fatty acid cycling rate, which is important in the metabolic regulation and heat production.

Amplite^® Colorimetric Glycerol Assay Kit offers a sensitive assay for measuring glycerol levels in biological samples. This assay is based on an enzyme coupled reaction of glycerol, in which the product hydrogen peroxide can be detected using Amplite^® Red substrate in the HRP-coupled reactions. The signal can be measured with an absorbance microplate reader using OD ratio of 570 nm/610 nm. With Amplite^® Colorimetric Glycerol Assay Kit, as low as 0.15 µg/mL (~1.6 µM) glycerol was detected in a 100 µL reaction volume.

Amplite^® Fluorimetric Glycerol Assay Kit is also based on an enzyme coupled reaction of glycerol, in which the product hydrogen peroxide can be detected using Amplite^® Red substrate in HRP-coupled reactions. The fluorescence signal can be measured using a fluorescence microplate reader at Ex/Em= 540/590 nm. With Amplite^® Fluorimetric Glycerol Assay Kit, as low as 0.015 µg/mL (~0.16 µM) glycerol was detected in a 100 µL reaction volume.