Multiplex Apoptosis and Necrosis

Apoptosis is an active, programmed process of autonomous cellular dismantling that avoids eliciting inflammation. In apoptosis, phosphatidylserine (PS) is transferred to the outer leaflet of the plasma membrane. As a universal indicator of the initial/intermediate stages of cell apoptosis, the appearance of phosphatidylserine on the cell surface can be detected before morphological changes are observed.

Necrosis is characterized as a passive, accidental cell death resulting from environmental perturbations with uncontrolled release of inflammatory cellular contents. Loss of plasma membrane integrity represents a straightforward approach to demonstrate late stage apoptosis and necrosis.

AAT Bioquest offers Cell Meter™ Apoptotic and Necrotic Detection Kits as a set of tools for monitoring cell viability. Our Cell Meter™ detection kits are optimized to simultaneously detect cell apoptosis, necrosis and healthy cells with a flow cytometer or fluorescence microscope. The phosphatidylserine (PS) sensor used in Kit 22843 has deep red fluorescence (Ex/Em = 630/660 nm) upon binding to membrane PS. Membrane-impermeable Nuclear Green™ DCS1 (Ex/Em = 490/525 nm) is used to label the nucleus while CytoCalcein™ Violet 450 (Ex/Em = 405/450 nm) is provided for labeling live cell cytoplasm.

Key Features of Cell Meter™ Detection Kits:

• Multiplexing capability, triple colors for the simultaneous detection of multiple cellular events.
• Robust, a mix and read format.
• Convenient, compatible with common filter sets.