Detect Calcium by Absorbance or Fluorescence

Calcium is essential for all living organisms, particularly in cell physiology. Calcium also plays an important role in mediating the constriction and relaxation of blood vessels, nerve impulse transmission, muscle contraction, and hormone secretion.

Amplite^® Fluorimetric Calcium Quantitation Kit (Cat. #36360) provides a simple method for detecting calcium in physiological solutions by using our proprietary red fluorescence probe. The fluorescence signal can be easily read with a fluorescence microplate reader at Ex/Em = 540/590 nm. With the Amplite^® Fluorimetric Calcium Quantitation Kit, we have detected as little as 0.03 mM calcium. The kit has a broad dynamic range (30 µM to 10 mM). Amplite^® Colorimetric Calcium Quantitation Kit (Cat. #36361) uses our Calcium Blue™ as the chromogenic calcium indicator. Its absorbance changes in response to calcium binding. Calcium Blue™ binds calcium tightly in the neutral pH range, generating a Calcium Blue™-calcium complex that has intense absorption at ~650 nm.