Z-DEVD-ProRed™ 620

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Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Shipping	Standard overnight for United States, inquire for international

Physical properties

Molecular weight	1565.50
Solvent	DMSO

Spectral properties

Excitation (nm)	532
Emission (nm)	619

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

Overview SDS Protocol

Example protocol

AT A GLANCE

Important notes
It is important to store at <-15 °C and should be stored in cool, dark place.

It can be used within 12 months from the date of receipt.

PREPARATION OF STOCK SOLUTION

Unless otherwise noted, all unused stock solutions should be divided into single-use aliquots and stored at -20 °C after preparation. Avoid repeated freeze-thaw cycles.

1. Z-DEVD-Prored^TM 620 stock solution (10 mM):
Add 65 µL of DMSO into the vial of 1 mg Z-DEVD-Prored^TM 620 to make 10 mM stock solution.

PREPARATION OF WORKING SOLUTION

Caspase 3/7 assay solution (2X):
Mix 50 µL Z-DEVD-ProRed™ 620 stock solution (10 mM), 100 µL DTT (1M), 400 µL EDTA (100 mM) and 10 mL Tris Buffer (20 mM), pH =7.4.

SAMPLE EXPERIMENTAL PROTOCOL

Mix equal volume of the caspase 3/7 standards or samples with 2X caspase 3/7 assay reaction solution and incubate at room temperature for at least 1 hour.
Monitor the fluorescence increase at Ex/Em = 535/620 nm.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Z-DEVD-ProRed™ 620 to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	63.877 µL	319.387 µL	638.774 µL	3.194 mL	6.388 mL
5 mM	12.775 µL	63.877 µL	127.755 µL	638.774 µL	1.278 mL
10 mM	6.388 µL	31.939 µL	63.877 µL	319.387 µL	638.774 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
	/		=		x		=

Spectrum

Open in Advanced Spectrum Viewer

Spectral properties

Excitation (nm)	532
Emission (nm)	619

Product Family

Name	Excitation (nm)	Emission (nm)
Z-IETD-ProRed™ 620	532	619
Z-LEHD-ProRed™ 620	532	619

Images

Figure 1. Detection of Caspase 3/7 Activities in Jurkat cells.
Jurkat cells were seeded on the same day at 200,000 cells/90 uL/well in a Costar black wall/clear bottom 96-well plate. The cells were treated with staurosporine at the final concentration of 1 uM for 5 hours while the untreated cells were used as control. The Z-DEVD-ProRed™ 620 assay solution (100 uL/well) was added and incubated at room temperature for 1 hour. The fluorescence intensity was measured at Ex/Em = 540/620 nm with FlexStation fluorescence microplate reader (Molecular Devices).

Citations

View all 2 citations: Citation Explorer

Degenerin channel activation causes caspase-mediated protein degradation and mitochondrial dysfunction in adult C. elegans muscle
Authors: Gaffney, Christopher J and Shephard, Freya and Chu, Jeff and Baillie, David L and Rose, Ann and Constantin-Teodosiu, Dumitru and Greenhaff, Paul L and Szewczyk, Nathaniel J
Journal: Journal of cachexia, sarcopenia and muscle (2016): 181--192

References

View all 101 references: Citation Explorer

Fisetin induces apoptosis in human cervical cancer HeLa cells through ERK1/2-mediated activation of caspase-8-/caspase-3-dependent pathway
Authors: Ying TH, Yang SF, Tsai SJ, Hsieh SC, Huang YC, Bau DT, Hsieh YH.
Journal: Arch Toxicol (2012): 263

Andrographolide induces apoptosis in B16F-10 melanoma cells by inhibiting NF-kappaB-mediated bcl-2 activation and modulating p53-induced caspase-3 gene expression
Authors: Pratheeshkumar P, Sheeja K, Kuttan G.
Journal: Immunopharmacol Immunotoxicol (2012): 143

ECRG4 is a negative regulator of caspase-8-mediated apoptosis in human T-leukemia cells
Authors: Matsuzaki J, Torigoe T, Hirohashi Y, Kamiguchi K, Tamura Y, Tsukahara T, Kubo T, Takahashi A, Nakazawa E, Saka E, Yasuda K, Takahashi S, Sato N.
Journal: Carcinogenesis (2012): 996

Proteasome inhibition can impair caspase-8 Activation upon sub-maximal Stimulation of apoptotic tumour necrosis factor-related apoptosis inducing ligand (TRAIL) signalling
Authors: Laussmann MA, Passante E, Hellwig CT, Tomiczek B, Flanagan L, Prehn JH, Huber HJ, Rehm M.
Journal: J Biol Chem. (2012)

Caspase-2 is an initiator caspase responsible for pore-forming toxin-mediated apoptosis
Authors: Imre G, Heering J, Takeda AN, Husmann M, Thiede B, Zu Heringdorf DM, Green DR, van der Goot FG, Sinha B, Dotsch V, Rajalingam K.
Journal: EMBO J. (2012)

Inhibitory effects of Scutellaria baicalensis extract on hepatic stellate cells through inducing G2/M cell cycle arrest and activating ERK-dependent apoptosis via Bax and caspase pathway
Authors: Pan TL, Wang PW, Leu YL, Wu TH, Wu TS.
Journal: J Ethnopharmacol (2012): 829

Berberine inhibits norepinephrine-induced apoptosis in neonatal rat cardiomyocytes via inhibiting ROS-TNF-alpha-caspase signaling pathway
Authors: Lv XX, Yu XH, Wang HD, Yan YX, Wang YP, Lu DX, Qi RB, Hu CF, Li HM.
Journal: Chin J Integr Med. (2012)

Imaging apoptosis with positron emission tomography: 'bench to bedside' development of the caspase-3/7 specific radiotracer [(18)F]ICMT-11
Authors: Nguyen QD, Challapalli A, Smith G, Fortt R, Aboagye EO.
Journal: Eur J Cancer (2012): 432

Musca domestica pupae lectin induces apoptosis in HepG2 cells through a NF-kappaB/p65-mediated caspase pathway
Authors: Nie J, Cao X, Zhou M, Zhang X, Zhang R, Niu L, Xia Y, Hou L, Wang C.
Journal: Bull Cancer (2012): 49

Caspase cleavage of viral proteins, another way for viruses to make the best of apoptosis
Authors: Richard A, Tulasne D.
Journal: Cell Death Dis (2012): e277