XFD660 Maleimide

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Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Shipping	Standard overnight for United States, inquire for international

Physical properties

Molecular weight	1016.09
Solvent	DMSO

Spectral properties

Extinction coefficient (cm ^-1 M ^-1)	132000
Excitation (nm)	663
Emission (nm)	691

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Direct upgrades

Overview SDS Protocol

Upgrade: iFluor® 660 maleimide

Molecular weight

1016.09

Extinction coefficient (cm ^-1 M ^-1)

132000

Excitation (nm)

663

Emission (nm)

691

XFD660, synthesized by AAT Bioquest, is chemically identical to Alexa Fluor® 660 (a ThermoFisher trademark). Designed for optimal excitation at 633 or 647 nm laser lines, XFD660 is well-suited for advanced imaging and flow cytometry applications, offering excellent fluorescence brightness and photostability. This dye is water-soluble and demonstrates pH stability within a range from 4 to 10. The maleimide derivative of XFD660 is commonly utilized for conjugation to thiol groups on proteins, oligonucleotide thiophosphates, or low molecular weight ligands, producing conjugates that exhibit significantly brighter fluorescence and enhanced photostability compared to those formed with other spectrally similar fluorophores.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of XFD660 Maleimide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	98.416 µL	492.082 µL	984.165 µL	4.921 mL	9.842 mL
5 mM	19.683 µL	98.416 µL	196.833 µL	984.165 µL	1.968 mL
10 mM	9.842 µL	49.208 µL	98.416 µL	492.082 µL	984.165 µL

Molarity calculator

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Spectrum

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Spectral properties

Extinction coefficient (cm ^-1 M ^-1)	132000
Excitation (nm)	663
Emission (nm)	691

Images

References

View all 37 references: Citation Explorer

A decade of blood-brain barrier permeability assays: Revisiting old traumatic brain injury rat data for new insights and experimental design.
Authors: Bolden, Chris T and Olson, Scott D and Cox, Charles S
Journal: Microvascular research (2023): 104453

A novel anti-TNF-α drug ozoralizumab rapidly distributes to inflamed joint tissues in a mouse model of collagen induced arthritis.
Authors: Oyama, Shohei and Ebina, Kosuke and Etani, Yuki and Hirao, Makoto and Kyuuma, Masanao and Fujii, Yasuyuki and Iwata, Katsuya and Ogawa, Bunichiro and Hasegawa, Tomoya and Kawano, Sasagu and Nakanishi, Yutaka and Okada, Seiji and Nakata, Ken
Journal: Scientific reports (2022): 18102

CD38-specific nanobodies allow in vivo imaging of multiple myeloma under daratumumab therapy.
Authors: Pape, Luca Julius and Hambach, Julia and Gebhardt, Anna Josephine and Rissiek, Björn and Stähler, Tobias and Tode, Natalie and Khan, Cerusch and Weisel, Katja and Adam, Gerhard and Koch-Nolte, Friedrich and Bannas, Peter
Journal: Frontiers in immunology (2022): 1010270

A 15-min non-competitive homogeneous assay for microcystin and nodularin based on time-resolved Förster resonance energy transfer (TR-FRET).
Authors: Akter, Sultana and Lamminmäki, Urpo
Journal: Analytical and bioanalytical chemistry (2021): 6159-6170

Alpha-MSH Targeted Liposomal Nanoparticle for Imaging in Inflammatory Bowel Disease (IBD).
Authors: Peñate-Medina, Tuula and Damoah, Christabel and Benezra, Miriam and Will, Olga and Kairemo, Kalevi and Humbert, Jana and Sebens, Susanne and Peñate-Medina, Oula
Journal: Current pharmaceutical design (2020): 3840-3846

Near-Infrared In Vivo Whole-Body Fluorescence Imaging of PNA.
Authors: Lim, Ernest Wee Kiat and Brolin, Camilla and Nielsen, Peter E
Journal: Methods in molecular biology (Clifton, N.J.) (2020): 251-260

CD24-targeted fluorescence imaging in patient-derived xenograft models of high-grade serous ovarian carcinoma.
Authors: Kleinmanns, Katrin and Bischof, Katharina and Anandan, Shamundeeswari and Popa, Mihaela and Akslen, Lars A and Fosse, Vibeke and Karlsen, Ida Tveit and Gjertsen, Bjørn T and Bjørge, Line and McCormack, Emmet
Journal: EBioMedicine (2020): 102782

A new class of recombinant human albumin with multiple surface thiols exhibits stable conjugation and enhanced FcRn binding and blood circulation.
Authors: Schelde, Karen Kræmmer and Nicholls, Karl and Dagnæs-Hansen, Frederik and Bunting, Karen and Rawsthorne, Helen and Andersen, Birgitte and Finnis, Christopher J A and Williamson, Miranda and Cameron, Jason and Howard, Kenneth A
Journal: The Journal of biological chemistry (2019): 3735-3743

Tracking Antibody Distribution with Near-Infrared Fluorescent Dyes: Impact of Dye Structure and Degree of Labeling on Plasma Clearance.
Authors: Cilliers, Cornelius and Nessler, Ian and Christodolu, Nikolas and Thurber, Greg M
Journal: Molecular pharmaceutics (2017): 1623-1633

Targetability of hyaluronic acid nanogel to cancer cells: In vitro and in vivo studies.
Authors: Pedrosa, S S and Pereira, P and Correia, A and Gama, F M
Journal: European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences (2017): 102-113